Research

Cell signalling pathways, Extracellular signals, Artificial Intelligence, Signalling heterogeneity in cancer

Interests

He is interested in understanding how cell signalling pathways driven by the activity of protein kinases contribute to the development of cancer. His research group is now using these unique resources to investigate the fundamental properties of signalling networks and to understand how signalling heterogeneity in cancer (with particular focus on haematological malignancies) contribute to intrinsic and acquired resistance to compounds that target signalling enzymes.

Publications

Publications of specific relevance to Applied Data Science

2021

Gerdes H, Casado P, Dokal A, Hijazi M, Akhtar N, Osuntola R, Rajeeve V, Fitzgibbon J, Travers J, Britton D, Khorsandi S and Cutillas PR (2021). Drug ranking using machine learning systematically predicts the efficacy of anti-cancer drugs. Nature Communications  vol. 12, (1) 10.1038/s41467-021-22170-8

Gualtieri A, Kyprianou N, Gregory LC, Vignola ML, Nicholson JG, Tan R, Inoue SI, Scagliotti V, Casado P, Blackburn J, Abollo-Jimenez F, Marinelli E, Besser REJ, Högler W, Karen Temple I, Davies JH, Gagunashvili A, Robinson ICAF, Camper SA, Davis SW, Cutillas PR, Gevers EF, Aoki Y, Dattani MT and Gaston-Massuet C (2021). Activating mutations in BRAF disrupt the hypothalamo-pituitary axis leading to hypopituitarism in mice and humans. Nature Communications  vol. 12, (1) 10.1038/s41467-021-21712-4

(2021). KDM5 inhibition offers a novel therapeutic strategy for the treatment of KMT2D mutant lymphomas. Blood  10.1182/blood.2020008743

Martín-Guerrero SM, Alonso P, Iglesias A, Cimadevila M, Brea J, Loza MI, Casado P, Martín-Oliva D, Cutillas PR, González-Maeso J and López-Giménez JF (2021). His452Tyr polymorphism in the human 5-HT2A receptor affects clozapine-induced signaling networks revealed by quantitative phosphoproteomics. Biochem Pharmacol  vol. 185, 10.1016/j.bcp.2021.114440

Bradley D, Viéitez C, Rajeeve V, Selkrig J, Cutillas PR and Beltrao P (2021). Sequence and Structure-Based Analysis of Specificity Determinants in Eukaryotic Protein Kinases. Cell Rep  vol. 34, (2) 10.1016/j.celrep.2020.108602

Freire-Benéitez V, Pomella N, Millner TO, Dumas AA, Niklison-Chirou MV, Maniati E, Wang J, Rajeeve V, Cutillas P and Marino S (2021). Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma. Nar Cancer  vol. 3, (1) 10.1093/narcan/zcab009

Edelmann J, Dokal AD, Vilventhraraja E, Holzmann K, Britton D, Klymenko T, Döhner H, Cragg M, Braun A, Cutillas P and Gribben JG (2021). Rituximab and obinutuzumab differentially hijack the B-cell receptor and NOTCH1 signaling pathways. Iscience  102089-102089. 10.1016/j.isci.2021.102089

2020

Martín-Guerrero SM, Casado P, Hijazi M, Rajeeve V, Plaza-Díaz J, Abadía-Molina F, Navascués J, Cuadros MA, Cutillas PR and Martín-Oliva D (2020). PARP-1 activation after oxidative insult promotes energy stress-dependent phosphorylation of YAP1 and reduces cell viability. Biochem J  vol. 477, (23) 4491-4513. 10.1042/BCJ20200525

Patel A, Jones T, Woodward L, Dokal A, Rajeeve V, Cutillas P, Stone T, Jacques T and Sheer D (2020). LGG-57. SIGNALLING MECHANISMS IN PAEDIATRIC LOW-GRADE GLIOMA. Neuro-Oncology  vol. 22, (Suppl 3) iii377-iii377. 10.1093/neuonc/noaa222.435

Patani H, Rushton MD, Higham J, Teijeiro SA, Oxley D, Cutillas P, Sproul D and Ficz G (2020). Transition to naïve human pluripotency mirrors pan-cancer DNA hypermethylation. Nature Communications  vol. 11, (1) 10.1038/s41467-020-17269-3

Rio-Machin A, Casado-Izquierdo P, Miettinen J, Bewicke-Copley F, Khan N, Demeulemeester J, Krizsán S, Zheng J, Middleton C, Yin W, Grey W, Miraki-Moud F, Matthews J, Grantham M, Rouault-Pierre K, Wennerberg K, Parsons A, Armes H, Close KE, Di Bella D, Cavenagh J, Freeman SD, Van Loo P, Bödör C, Porkka K, Heckman CA, Wang J, Taussig D, Bonnet D and Cazier J-B (2020). Integration of Deep Multi-Omics Profiling Veals New Insights into the Biology of Poor-Risk Acute Myeloid Leukemia. Blood  vol. 136, (Supplement 1) 39-40. 10.1182/blood-2020-141345

Fernández-Varo G, Perramón M, Carvajal S, Oró D, Casals E, Boix L, Oller L, Macías-Muñoz L, Marfà S, Casals G, Morales-Ruiz M, Casado P, Cutillas PR, Bruix J, Navasa M, Fuster J, Garcia-Valdecasas JC, Pavel MC, Puntes V and Jiménez W (2020). Bespoken Nanoceria: An Effective Treatment in Experimental Hepatocellular Carcinoma. Hepatology  vol. 72, (4) 1267-1282. 10.1002/hep.31139

Xu R, Jones W, Wilcz-Villega E, Costa ASH, Rajeeve V, Bentham RB, Bryson K, Nagano A, Yaman B, Olendo Barasa S, Wang Y, Chelala C, Cutillas P, Szabadkai G, Frezza C and Bianchi K (2020). The breast cancer oncogene IKKε coordinates mitochondrial function and serine metabolism. Embo Reports  vol. 21, (9) 10.15252/embr.201948260

Akhtar N, Waleed Baig M, Haq IU, Rajeeve V and Rodriguez Cutillas P (2020). Withanolide metabolites inhibit PI3K/AKT and MAPK pro-survival pathways and induce apoptosis in acute myeloid leukemia cells. Biomedicines  vol. 8, (9) 10.3390/biomedicines8090333

Gadaleta E, Fourgoux P, Pirró S, Thorn GJ, Nelan R, Ironside A, Rajeeve V, Cutillas PR, Lobley AE, Wang J, Gea E, Ross-Adams H, Bessant C, Lemoine NR, Jones LJ and Chelala C (2020). Characterization of four subtypes in morphologically normal tissue excised proximal and distal to breast cancer. Nature Research (Part of Springer Nature)  Npj Breast Cancer  vol. 6, (1) 10.1038/s41523-020-00182-9

Gadaleta E, Fourgoux P, Pirró S, Thorn GJ, Nelan R, Ironside A, Rajeeve V, Cutillas PR, Lobley AE, Wang J, Gea E, Ross-Adams H, Bessant C, Lemoine NR, Jones LJ and Chelala C (2020). Characterization of four subtypes in morphologically normal tissue excised proximal and distal to breast cancer. Npj Breast Cancer  vol. 6, (1) 10.1038/s41523-020-00182-9

Kostaras E, Kaserer T, Lazaro G, Heuss SF, Hussain A, Casado P, Hayes A, Yandim C, Palaskas N, Yu Y, Schwartz B, Raynaud F, Chung YL, Cutillas PR and Vivanco I (2020). A systematic molecular and pharmacologic evaluation of AKT inhibitors reveals new insight into their biological activity. British Journal of Cancer  vol. 123, (4) 542-555. 10.1038/s41416-020-0889-4

Wong PP, Muñoz-Félix JM, Hijazi M, Kim H, Robinson SD, De Luxán-Delgado B, Rodríguez-Hernández I, Maiques O, Meng YM, Meng Q, Bodrug N, Dukinfield MS, Reynolds LE, Elia G, Clear A, Harwood C, Wang Y, Campbell JJ, Singh R, Zhang P, Schall TJ, Matchett KP, Henderson NC, Szlosarek PW, Dreger SA, Smith S, Jones JL, Gribben JG, Cutillas PR and Meier P (2020). Cancer Burden Is Controlled by Mural Cell-β3-Integrin Regulated Crosstalk with Tumor Cells. Cell  vol. 181, (6) 1346-1363.e21. 10.1016/j.cell.2020.02.003

Invergo BM, Petursson B, Akhtar N, Bradley D, Giudice G, Hijazi M, Cutillas P, Petsalaki E and Beltrao P (2020). Prediction of Signed Protein Kinase Regulatory Circuits. Cell Syst  vol. 10, (5) 384-396.e9. 10.1016/j.cels.2020.04.005

Demircioglu F, Wang J, Candido J, Costa ASH, Casado P, de Luxan Delgado B, Reynolds LE, Gomez-Escudero J, Newport E, Rajeeve V, Baker A-M, Roy-Luzarraga M, Graham TA, Foster J, Wang Y, Campbell JJ, Singh R, Zhang P, Schall TJ, Balkwill FR, Sosabowski J, Cutillas PR, Frezza C, Sancho P and Hodivala-Dilke K (2020). Cancer associated fibroblast FAK regulates malignant cell metabolism. Nature Research (Part of Springer Nature)  Nature Communications  vol. 11, (1) 1290-1290. 10.1038/s41467-020-15104-3

Huang KW, Reebye V, Czysz K, Ciriello S, Dorman S, Reccia I, Lai HS, Peng L, Kostomitsopoulos N, Nicholls J, Habib RS, Tomalia DA, Sætrom P, Wilkes E, Cutillas P, Rossi JJ and Habib NA (2020). Liver Activation of Hepatocellular Nuclear Factor-4α by Small Activating RNA Rescues Dyslipidemia and Improves Metabolic Profile. Molecular Therapy - Nucleic Acids  vol. 19, 361-370. 10.1016/j.omtn.2019.10.044

Hijazi M, Smith R, Rajeeve V, Bessant C and Cutillas PR (2020). Reconstructing kinase network topologies from phosphoproteomics data reveals cancer-associated rewiring. Nature Research  Nature Biotechnology  10.1038/s41587-019-0391-9

2019

Grey W, Casado-Izquierdo P, Cutillas P and Bonnet D (2019). Combination Therapy Targeting CKS1-Dependent Protein Degradation Reduces AML Burden Whilst Protecting Normal Haematopoietic Stem Cellsfrom Cytarabine Toxicity. Blood  vol. 134, (Supplement_1) 2535-2535. 10.1182/blood-2019-121667

Edelmann J, Dokal A, Holzmann K, Britton DJ, Vilventhraraja E, Smith RJ, Cragg MS, Braun A, Döhner H, Cutillas P and Gribben JG (2019). Rituximab and Obinutuzumab Induce Direct B-Cell Death Via B-Cell Receptor (BCR) Signaling, but Rituximab Elicits Stronger BCR-Derived Pro-Survival Signals Diminishing Apoptosis. Blood  vol. 134, (Supplement_1) 1579-1579. 10.1182/blood-2019-128704

Martín-Guerrero SM, Casado P, Muñoz-Gámez JA, Carrasco M-C, Navascués J, Cuadros MA, López-Giménez JF, Cutillas PR and Martín-Oliva D (2019). Poly(ADP-Ribose) Polymerase-1 inhibition potentiates cell death and phosphorylation of DNA damage response proteins in oxidative stressed retinal cells. Exp Eye Res  vol. 188, 10.1016/j.exer.2019.107790

Alrawashdeh W, Jones R, Dumartin L, Radon TP, Cutillas PR, Feakins RM, Dmitrovic B, Demir IE, Ceyhan GO and Crnogorac-Jurcevic T (2019). Perineural invasion in pancreatic cancer: proteomic analysis and in vitro modelling. Molecular Oncology  vol. 13, (5) 1075-1091. 10.1002/1878-0261.12463

Kostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S, Cutillas P and Nightingale TD (2019). Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration. Plos Biology  vol. 17, (12) 10.1371/journal.pbio.3000554

2018

Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR and Hynes RO (2018). Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics. Journal of Proteome Research  vol. 16, (8) 3083-3091. 10.1021/acs.jproteome.7b00191

Ferrandiz N, Barroso C, Telecan O, Shao N, Kim HM, Testori S, Faull P, Cutillas P, Snijders AP, Colaiácovo MP and Martinez-Perez E (2018). Erratum to: Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis (Nature Communications, (2018), 9, 1, (834), 10.1038/s41467-018-03229-5). Nature Communications  vol. 9, (1) 10.1038/s41467-018-05848-4

Ferrandiz N, Barroso C, Telecan O, Shao N, Kim HM, Testori S, Faull P, Cutillas P, Snijders AP, Colaiácovo MP and Martinez-Perez E (2018). Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. Elegans oocyte meiosis. Nature Communications  vol. 9, (1) 10.1038/s41467-018-03229-5

Arnandis T, Monteiro P, Adams SD, Bridgeman VL, Rajeeve V, Gadaleta E, Marzec J, Chelala C, Malanchi I, Cutillas PR and Godinho SA (2018). Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion. Developmental Cell  vol. 47, (4) 409-424.e9. 10.1016/j.devcel.2018.10.026

Angulo-Urarte A, Casado P, Castillo SD, Kobialka P, Kotini MP, Figueiredo AM, Castel P, Rajeeve V, Milà-Guasch M, Millan J, Wiesner C, Serra H, Muixi L, Casanovas O, Viñals F, Affolter M, Gerhardt H, Huveneers S, Belting H-G, Cutillas PR and Graupera M (2018). Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility. Nat Commun  vol. 9, (1) 10.1038/s41467-018-07172-3

Simon T, Pinioti S, Schellenberger P, Rajeeve V, Wendler F, Cutillas PR, King A, Stebbing J and Giamas G (2018). Shedding of bevacizumab in tumour cells-derived extracellular vesicles as a new therapeutic escape mechanism in glioblastoma. Mol Cancer  vol. 17, (1) 10.1186/s12943-018-0878-x

Casado P, Wilkes EH, Miraki-Moud F, Hadi MM, Rio-Machin A, Rajeeve V, Pike R, Iqbal S, Marfa S, Lea N, Best S, Gribben J, Fitzgibbon J and Cutillas PR (2018). Proteomic and genomic integration identifies kinase and differentiation determinants of kinase inhibitor sensitivity in leukemia cells. Leukemia  vol. 32, (8) 1818-1822. 10.1038/s41375-018-0032-1

Tummala H, Dokal AD, Walne A, Ellison A, Cardoso S, Amirthasigamanipillai S, Kirwan M, Browne I, Sidhu JK, Rajeeve V, Rio-Machin A, Seraihi AA, Duncombe AS, Jenner M, Smith OP, Enright H, Norton A, Aksu T, Özbek NY, Pontikos N, Cutillas P, Dokal I and Vulliamy T (2018). Genome instability is a consequence of transcription deficiency in patients with bone marrow failure harboring biallelic ERCC6L2 variants. Proc Natl Acad Sci U S A  vol. 115, (30) 7777-7782. 10.1073/pnas.1803275115

Fearon AE, Carter EP, Clayton NS, Wilkes EH, Baker AM, Kapitonova E, Bakhouche BA, Tanner Y, Wang J, Gadaleta E, Chelala C, Moore KM, Marshall JF, Chupin J, Schmid P, Jones JL, Lockley M, Cutillas PR and Grose RP (2018). PHLDA1 Mediates Drug Resistance in Receptor Tyrosine Kinase-Driven Cancer. Cell Reports  vol. 22, (9) 2469-2481. 10.1016/j.celrep.2018.02.028

2017

Casado P, Wilkes EH, Miraki-Moud F, Hadi MM, Rio-Machin A, Rajeeve V, Pike R, Iqbal S, Marfa S, Lea N, Best S, Gribben J, Fitzgibbon J and Cutillas PR (2017). Proteomic and genomic integration identifies kinase and differentiation determinants of kinase inhibitor sensitivity in leukemia cells. Leukemia  10.1038/leu.2017.349

Pearce OMT, Delaine-Smith R, Maniati E, Nichols S, Wang J, Böhm S, Rajeeve V, Ullah D, Chakravarty P, Jones RR, Montfort A, Dowe T, Gribben J, Jones JL, Kocher HM, Serody JS, Vincent BG, Connelly J, Brenton JD, Chelala C, Cutillas PR, Lockley M, Bessant C, Knight M and Balkwill FR (2017). Deconstruction of a metastatic tumor microenvironment reveals a common matrix response in human cancers. Cancer Discov  10.1158/2159-8290.CD-17-0284

Kyprianou N, Gregory L, Vignola ML, Marinelli E, Gualtieri A, Scagliotti V, Davis S, Casado P, Rajeeve V, Cutillas P, Gevers E, Dattani M and Gaston-Massuet C (2017). The MAPK effector B-Raf is essential for hypothalamic-pituitary axis development and activating mutations in BRAF Cause Congenital hypopituitarism. Endocrine Abstracts  10.1530/endoabs.51.oc4.4

Dermit M, Dokal A and Cutillas PR (2017). Approaches to identify kinase dependencies in cancer signalling networks. Febs Lett  vol. 591, (17) 2577-2592. 10.1002/1873-3468.12748

Wilkes EH, Casado P, Rajeeve V and Cutillas PR (2017). Kinase activity ranking using phosphoproteomics data (KARP) quantifies the contribution of protein kinases to the regulation of cell viability. Mol Cell Proteomics  vol. 16, (9) 1694-1704. 10.1074/mcp.O116.064360

Wilkes E and Cutillas PR (2017). Label-Free Phosphoproteomic Approach for Kinase Signaling Analysis. Springerlink  Methods Mol Biol  vol. 1636, 199-217. 10.1007/978-1-4939-7154-1_13

Yuzugulen J, Douthwaite JA, Wood EG, Villar IC, Patel NSA, Jegard J, Gaertner H, Rossitto-Borlat I, Rose K, Hartley O, Cutillas PR, Ahluwalia A and Corder R (2017). Characterisation of preproendothelin-1 derived peptides identifies Endothelin-Like Domain Peptide as a modulator of Endothelin-1. Sci Rep  vol. 7, (1) 4956-4956. 10.1038/s41598-017-05365-2

Cutillas PR (2017). Targeted In-Depth Quantification of Signaling Using Label-Free Mass Spectrometry. Methods Enzymol  vol. 585, 245-268. 10.1016/bs.mie.2016.09.021

2016

Dermit M, Casado P, Rajeeve V, Wilkes EH, Foxler DE, Campbell H, Critchlow S, Sharp TV, Gribben JG, Unwin R and Cutillas PR (2016). Oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells resistant to PI3K inhibition. Nature Publishing Group  Oncogene  10.1038/onc.2016.435

Afzal TA, Luong LA, Chen D, Zhang C, Yang F, Chen Q, An W, Wilkes E, Yashiro K, Cutillas PR, Zhang L and Xiao Q (2016). NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia. Wiley  Journal of The American Heart Association  vol. 5, (12) e004629-e004629. 10.1161/JAHA.116.004629

Villoria MT, Ramos F, Dueñas E, Faull P, Cutillas PR and Clemente-Blanco A (2016). Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair. Embo Press  Embo J  vol. 36, (1) 79-101. 10.15252/embj.201593540

Scotcher J, Prysyazhna O, Boguslavskyi A, Kistamas K, Hadgraft N, Martin ED, Worthington J, Rudyk O, Rodriguez Cutillas P, Cuello F, Shattock MJ, Marber MS, Conte MR, Greenstein A, Greensmith DJ, Venetucci L, Timms JF and Eaton P (2016). Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. Nature Publishing Group: Nature Communications  Nature Communications  vol. 7, 13187-13187. 10.1038/ncomms13187

Casado P, Hijazi M, Britton D and Cutillas PR (2016). Impact of phosphoproteomics in the translation of kinase targeted therapies. Wiley-Vch Verlag  Proteomics  10.1002/pmic.201600235

Sciacovelli M, Gonçalves E, Johnson TI, Zecchini VR, da Costa ASH, Gaude E, Drubbel AV, Theobald SJ, Abbo SR, Tran MGB, Rajeeve V, Cardaci S, Foster S, Yun H, Cutillas P, Warren A, Gnanapragasam V, Gottlieb E, Franze K, Huntly B, Maher ER, Maxwell PH, Saez-Rodriguez J and Frezza C (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature  vol. 537, (7621) 544-547. 10.1038/nature19353

Montfort A, Pearce OMT, Maniati E, Vincent B, Bixby LM, Bo hm S, Dowe T, Wilkes EH, Chakravarty P, Thompson R, Topping J, Cutillas PR, Lockley M, Serody JS, Capasso M and Balkwill FR (2016). A strong B cell response is part of the immune landscape in human high-grade serous ovarian metastases. American Association For Cancer Research  Clinical Cancer Research  10.1158/1078-0432.CCR-16-0081

Kulbe H, Iorio F, Chakravarty P, Milagre CS, Moore R, Thompson RG, Everitt G, Canosa M, Montoya A, Drygin D, Braicu I, Sehouli J, Saez-Rodriguez J, Cutillas PR and Balkwill FR (2016). Integrated transcriptomic and proteomic analysis identifies protein kinase CK2 as a key signaling node in an inflammatory cytokine network in ovarian cancer cells. Oncotarget  vol. 7, (13) 15648-15661. 10.18632/oncotarget.7255

Quétier I, Marshall JJT, Spencer-Dene B, Lachmann S, Casamassima A, Franco C, Escuin S, Worrall JT, Baskaran P, Rajeeve V, Howell M, Copp AJ, Stamp G, Rosewell I, Cutillas P, Gerhardt H, Parker PJ and Cameron AJM (2016). Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion. Cell Reports  vol. 14, (3) 440-448. 10.1016/j.celrep.2015.12.049

Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Arozena AA, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Airoldi EM, Ait-Si-Ali S, Akematsu T, Akporiaye ET, Al-Rubeai M, Albaiceta GM, Albanese C, Albani D, Albert ML, Aldudo J and Algül H (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy  vol. 12, (1) 1-222. 10.1080/15548627.2015.1100356

Cutillas PR and Crnogorac-Jurcevic T (2016). Application of proteomics in cancer biomarker discovery: GeLC-MS/MS. 10.1007/978-1-4939-3204-7_11

2015

Terfve CDA, Wilkes EH, Casado P, Cutillas PR and Saez-Rodriguez J (2015). Large-scale models of signal propagation in human cells derived from discovery phosphoproteomic data. Nat Commun  vol. 6, 10.1038/ncomms9033

Carretero L, Llavona P, López-Hernández A, Casado P, Cutillas PR, de la Peña P, Barros F and Domínguez P (2015). ERK and RSK are necessary for TRH-induced inhibition of r-ERG potassium currents in rat pituitary GH3 cells. Cell Signal  vol. 27, (9) 1720-1730. 10.1016/j.cellsig.2015.05.014

Wilkes EH, Terfve C, Gribben JG, Saez-Rodriguez J and Cutillas PR (2015). Empirical inference of circuitry and plasticity in a kinase signaling network. Proceedings of The National Academy of Sciences of The United States of America  vol. 112, (25) 7719-7724. 10.1073/pnas.1423344112

Cutillas PR (2015). Role of phosphoproteomics in the development of personalized cancer therapies. Proteomics Clin Appl  vol. 9, (3-4) 383-395. 10.1002/prca.201400104

Hall AM, Vilasi A, Garcia-Perez I, Lapsley M, Alston CL, Pitceathly RDS, McFarland R, Schaefer AM, Turnbull DM, Beaumont NJ, Hsuan JJ, Cutillas PR, Lindon JC, Holmes E, Unwin RJ, Taylor RW, Gorman GS, Rahman S and Hanna MG (2015). The urinary proteome and metabonome differ from normal in adults with mitochondrial disease. Kidney Int  vol. 87, (3) 610-622. 10.1038/ki.2014.297

(2015). Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation. Mol Cell Proteomics  vol. 14, (3) 484-498. 10.1074/mcp.M114.043836

2014

Wilkes EH, Casado P and Cutillas PR (2014). Approaches for measuring signalling plasticity in the context of resistance to targeted cancer therapies. Biochem Soc Trans  vol. 42, (4) 791-797. 10.1042/BST20140029

Rajeeve V, Vendrell I, Wilkes E, Torbett N and Cutillas PR (2014). Cross-species proteomics reveals specific modulation of signaling in cancer and stromal cells by phosphoinositide 3-kinase (PI3K) inhibitors. Mol Cell Proteomics  vol. 13, (6) 1457-1470. 10.1074/mcp.M113.035204

Casado P, Bilanges B, Rajeeve V, Vanhaesebroeck B and Cutillas PR (2014). Environmental Stress Affects the Activity of Metabolic and Growth Factor Signaling Networks and Induces Autophagy Markers in MCF7 Breast Cancer Cells. Molecular & Cellular Proteomics  vol. 13, (3) 836-848. 10.1074/mcp.M113.034751

2013

Yuzugulen J, Wood EG, Villar IC, Douthwaite JA, Patel NSAI, Jegard J, Montoya A, Cutillas P, Gaertner H, Rossitto-Borlat I, Rose K, Hartley O, Ahluwalia A and Corder R (2013). Characterisation of the “Endothelin-Like Domain Peptide” (ELDP) co-synthesised with Endothelin-1 from the EDN1 gene. Life Sciences  vol. 93, (25-26) 10.1016/j.lfs.2013.12.037

Lumb S, Torbett N, Vendrell I, Turner H, Page M, Hales P, Maloney A, Vanhaesebroeck B, Cutillas P and Shock A (2013). THU0045 Epratuzumab, an Antibody Targeting CD22 on B Cells, Induces Phosphoprotein Changes Following B-Cell Receptor Activation in Vitro. Annals of The Rheumatic Diseases  vol. 72, (Suppl 3) 10.1136/annrheumdis-2013-eular.573

Casado P, Alcolea MP, Iorio F, Rodríguez-Prados J-C, Vanhaesebroeck B, Saez-Rodriguez J, Joel S and Cutillas PR (2013). Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol  vol. 14, (4) 10.1186/gb-2013-14-4-r37

Casado P, Rodriguez-Prados J-C, Cosulich SC, Guichard S, Vanhaesebroeck B, Joel S and Cutillas PR (2013). Kinase-substrate enrichment analysis provides insights into the heterogeneity of signaling pathway activation in leukemia cells. Sci Signal  vol. 6, (268) 10.1126/scisignal.2003573

Rajeeve V, Pearce W, Cascante M, Vanhaesebroeck B and Cutillas PR (2013). Polyamine production is downstream and upstream of oncogenic PI3K signalling and contributes to tumour cell growth. Biochem J  vol. 450, (3) 619-628. 10.1042/BJ20121525

Alrawashdeh W, Jones R, Cutillas P, Wijesuriya N and Crnogorac-Jurcevic T (2013). Proteomic analysis of perineural invasion in pancreatic adenocarcinoma reveals up-regulation of neurosecretory protein VGF in invaded nerves. Pancreatology  vol. 13, (2) 10.1016/j.pan.2012.12.066

2012

Beltran L, Casado P, Rodríguez-Prados J-C and Cutillas PR (2012). Global profiling of protein kinase activities in cancer cells by mass spectrometry. J Proteomics  vol. 77, 492-503. 10.1016/j.jprot.2012.09.029

Beltran L and Cutillas PR (2012). Advances in phosphopeptide enrichment techniques for phosphoproteomics. Amino Acids  vol. 43, (3) 1009-1024. 10.1007/s00726-012-1288-9

Cappadona S, Baker PR, Cutillas PR, Heck AJR and van Breukelen B (2012). Current challenges in software solutions for mass spectrometry-based quantitative proteomics. Amino Acids  vol. 43, (3) 1087-1108. 10.1007/s00726-012-1289-8

Alcolea MP, Casado P, Rodríguez-Prados J-C, Vanhaesebroeck B and Cutillas PR (2012). Phosphoproteomic analysis of leukemia cells under basal and drug-treated conditions identifies markers of kinase pathway activation and mechanisms of resistance. Mol Cell Proteomics  vol. 11, (8) 453-466. 10.1074/mcp.M112.017483

Kitromilidou C, McDonald D, Cutillas P, Ghazaly E, Perry J, Csaba B, Joel S and Powles T (2012). Abstract 1792: The potential role of targeting HER1-4 in bladder cancer. 10.1158/1538-7445.am2012-1792

Kulbe H, Chakravarty P, Moore R, Iorio F, Montoya A, Saez-Rodriguez J, Cutillas P and Balkwill F (2012). Abstract 49: Targeting the TNF network in human ovarian cancer - A systems biology approach. 10.1158/1538-7445.am2012-49

Burling KA, Cutillas PR, Church D, Lapsley M and Norden AGW (2012). Analysis of molecular forms of urine Retinol-Binding Protein in Fanconi Syndrome and design of an accurate immunoassay. Clinica Chimica Acta  vol. 413, (3-4) 483-489. 10.1016/j.cca.2011.11.007

2011

Worthington J, Cutillas PR and Timms JF (2011). IMAC/TiO2 enrich for peptide modifications other than phosphorylation: Implications for chromatographic choice and database searching in phosphoproteomics. Proteomics  vol. 11, (23) 4583-4587. 10.1002/pmic.201100143

Beltran L, Chaussade C, Vanhaesebroeck B and Cutillas PR (2011). Calpain interacts with class IA phosphoinositide 3-kinases regulating their stability and signaling activity. Proc Natl Acad Sci U S A  vol. 108, (39) 16217-16222. 10.1073/pnas.1107692108

Montoya A, Beltran L, Casado P, Rodríguez-Prados J-C and Cutillas PR (2011). Characterization of a TiO₂ enrichment method for label-free quantitative phosphoproteomics. Methods  vol. 54, (4) 370-378. 10.1016/j.ymeth.2011.02.004

Cutillas PR and Jørgensen C (2011). Biological signalling activity measurements using mass spectrometry. Biochem J  vol. 434, (2) 189-199. 10.1042/BJ20101974

Casado P and Cutillas PR (2011). A self-validating quantitative mass spectrometry method for assessing the accuracy of high-content phosphoproteomic experiments. Mol Cell Proteomics  vol. 10, (1) 10.1074/mcp.M110.003079

2010

Cutillas PR and Timms JF (2010). Approaches and applications of quantitative LC-MS for proteomics and activitomics. Methods Mol Biol  vol. 658, 3-17. 10.1007/978-1-60761-780-8_1

Alcolea MP and Cutillas PR (2010). In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry. Methods Mol Biol  vol. 658, 111-126. 10.1007/978-1-60761-780-8_6

Cutillas PR (2010). Analysis of peptides in biological fluids by LC-MS/MS. Methods Mol Biol  vol. 658, 311-321. 10.1007/978-1-60761-780-8_19

Alcolea MP and Cutillas PR (2010). Quantification of protein kinase activities by LC-MS. Methods Mol Biol  vol. 658, 325-337. 10.1007/978-1-60761-780-8_20

2009

Cutillas PR (2009). Quantifying oncogenic signalling by mass spectrometry. Amino Acids  vol. 37, (4) 762-762.

Alcolea MP, Kleiner O and Cutillas PR (2009). Increased confidence in large-scale phosphoproteomics data by complementary mass spectrometric techniques and matching of phosphopeptide data sets. J Proteome Res  vol. 8, (8) 3808-3815. 10.1021/pr800955n

Quintana LF, Campistol JM, Alcolea MP, Banon-Maneus E, Sole-Gonzalez A and Cutillas PR (2009). Application of Label-free Quantitative Peptidomics for the Identification of Urinary Biomarkers of Kidney Chronic Allograft Dysfunction. Mol Cell Proteomics  vol. 8, (7) 1658-1673. 10.1074/mcp.M900059-MCP200

2008

Vilasi A, Cutillas PR and Unwin RJ (2008). Application of proteomic techniques to the study of urine and renal tissue. Proteom Clin Appl  vol. 2, (12) 1564-1574. 10.1002/prca.200800035

Graupera M, Guillermet-Guibert J, Foukas LC, Phng LK, Cain RJ, Salpekar A, Pearce W, Meek S, Millan J, Cutillas PR, Smith AJH, Ridley AJ, Ruhrberg C, Gerhardt H and Vanhaesebroeck B (2008). Angiogenesis selectively requires the p110 alpha isoform of PI3K to control endothelial cell migration. Nature  vol. 453, (7195) 662-U9. 10.1038/nature06892

2007

Cutillas PR and Vanhaesebroeck B (2007). Quantitative profile of five murine core proteomes using label-free functional proteomics. Mol Cell Proteomics  vol. 6, (9) 1560-1573. 10.1074/mcp.M700037-MCP200

Vilasi A, Cutillas PR, Maher AD, Zirah SFM, Capasso G, Norden AWG, Holmes E, Nicholson JK and Unwin RJ (2007). Combined proteomic and metabonomic studies in three genetic forms of the renal Fanconi syndrome. Am J Physiol Renal Physiol  vol. 293, (2) F456-F467. 10.1152/ajprenal.00095.2007

Cutillas PR and Vanhaesebroeck B (2007). Profiling protein expression by label-free quantitative mass Spectrometry. Mol Cell Proteomics  vol. 6, (8) 14-14.

Geering B, Cutillas PR, Nock G, Gharbi SI and Vanhaesebroeck B (2007). Class IA phosphoinositide 3-kinases are obligate p85-p110 heterodimers. Proc Natl Acad Sci U S A  vol. 104, (19) 7809-7814. 10.1073/pnas.0700373104

VANHAESEBROECK BAM, Gharbi SL, Nock G, Geering B and Cutillas PR (2007). Class IA phosphoinositide 3-kinases are obligate p85-p110 heterodimers. Proc Natl Acad Sci U S A  vol. 104, 7809-7814. 10.1073/pnas.0700373104

Norden AGW, Rodriguez-Cutillas P and Unwin RJ (2007). Clinical urinary peptidomics: Learning to walk before we can run. Clinical Chemistry  vol. 53, (3) 375-376. 10.1373/clinchem.2006.084038

2006

CUTILLAS PR, Vanhaesebroeck BAM, Khwaja A, Waterfield M, Gharbi S, Pearce W and Graupera M (2006). Ultrasensitive and absolute quantification of the phosphoinositide 3-kinase/Akt signal transduction pathway by mass spectrometry. Proc Natl Acad Sci Usa  vol. 103, (24) 8959-8964. 10.1073/pnas.0602101103

2005

Cutillas PR, Geering B, Waterfield MD and Vanhaesebroeck B (2005). Quantification of gel-separated proteins and their phosphorylation sites by LC-MS using unlabeled internal standards: analysis of phosphoprotein dynamics in a B cell lymphoma cell line. Mol Cell Proteomics  vol. 4, (8) 1038-1051. 10.1074/mcp.M500078-MCP200

CUTILLAS PR, Biber J, Marks J, Waterfield M, Cramer R, Stieger B, Jacob R, Burlingame AL and Unwin RJ (2005). Proteomic analysis of plasma membrane vesicles isolated from the rat renal cortex. Proteomics  vol. 5, (1) 101-112. 10.1002/pmic.200400886

2004

CUTILLAS PR, Burlingame AL, Waterfield MD, Norden AG, Cramer R, Hansen KC, Chalkley RJ and Unwin RJ (2004). The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells. Am J Physiol Renal Physiol  vol. 287, (3) F353-F364. 10.1152/ajprenal.00018.2004